Table 4 Bacterial strains and plasmids used in this work

E. coli DH5α

F ⁻, endA1, hsdR17 (rk-mk-),supE44, thi1, recA1, gyrA, (Nalr), relA1, D(lacZYAargF), U169, and F80lacZDM15

Invitrogen

G. oxydans DSM2343

Wild-type, Cef^R

DSMZ^a

G. oxydans ZJU2

Gluconate 2-dehydrogenase and pyruvate decarboxylase deletion strain derived from G. oxydans DSM2343, Cef^R

[21]

G. oxydans ZJU3

G. oxydans ZJU2 harboring pBB5-P₀₁₆₉-sldAB, Cef^R, Gm^R

This work

G. oxydans ZJU4

G. oxydans ZJU3 harboring pUCpr-T1, Cef^R, Gm^R, Amp^R

This work

G. oxydans ZJU5

G. oxydans ZJU3 harboring pUCpr-T2, Cef^R, Gm^R, Amp^R

This work

G. oxydans ZJU6

G. oxydans ZJU3 harboring pUCpr-T3, Cef^R, Gm^R, Amp^R

This work

G. oxydans ZJU7

G. oxydans ZJU3 harboring pUCpr-T4, Cef^R, Gm^R, Amp^R

This work

pUC19

Cloning vector, ColE1 ori, Amp^R

Invitrogen

pET28 (a)-GFP

gpf gene expressed vector, Km^R

Laboratory preservation

pBBR1MCS-5

Broad-host-range (bhr) expression vector, Gm^R

[45]

pBB5-P_tufB

Insert P_tufB promoter vector derived from pBBR1MCS-5, Gm^R

This work

pBB5-P₀₂₆₄

Insert P₀₂₆₄ promoter vector derived from pBBR1MCS-5, Gm^R

This work

pBB5-P₀₄₅₂

Insert P₀₄₅₂ promoter vector derived from pBBR1MCS-5, Gm^R

This work

pBB5-P₀₁₆₉

Insert P₀₁₆₉ promoter vector derived from pBBR1MCS-5, Gm^R

This work

pBB5-P₀₁₆₉-sldAB

sldAB gene overexpression vector derived from pBBR1MCS-5, inserted P₀₁₆₉ promoter, Gm^R

This work

pUCpr

Constructed expression vector derived from pUC19, par-rep, Amp^R

This work

pUCpr-T1

pUCpr-P₀₁₆₉-pqqABCDE vector derived from pUCpr, Amp^R

This work

pUCpr-T2

pUCpr-P₀₁₆₉-pqqABCDE-tldD vector derived from pUCpr, Amp^R

This work

pUCpr-T3

pUCpr-P₀₁₆₉-pqqABCDE-P₀₁₆₉-cyoBACD vector derived from pUCpr, Amp^R

This work

pUCpr-T4

pUCpr-P₀₁₆₉-pqqABCDE-tldD-P₀₁₆₉-cyoBACD vector derived from pUCpr, Amp^R

This work