Table 3 Purification of LipC24 from E. coli Origami 2(DE3)-pEDSF-lipB-lipC24
Steps | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
Cell-free extract | 245.39 | 89.78 | 2.73 | 100 | 1 |
HisTrap HP | 57.96 | 1.8 | 32.20 | 23.62 | 11.79 |
HiTrap DEAE FF | 52.74 | 1.09 | 48.31 | 21.49 | 17.70 |