Table 2 Oligonucleotide primers used in the current studya
Primers | Oligonucleotide sequence (5’ to 3’) | Annealing temperature (°C) | PCR products |
|---|---|---|---|
lipACF | AAGGATCCTCGGCGTCGACAACGTGCTGAACAAG | 52 | Full length of lipA and the corresponding chaperonin lipB |
lipACR | CGAAAGCTTCGCCAACACCATCGAGCAACATCTG | ||
lipC21CF | TCGATGGCTTGGGTGACGGACA | 59 | Full length of lipC21 |
lipC21CR | CGAAGTTGGCTGGCACTCTTTGGC | ||
lipC24CF | CTAGTGCAGCGTCTCGGGCGCGA | 62 | Full length of lipC24 |
lipC24CR | CACCATGTCCTCCAGACGTTTCATGATGG | ||
lipBEF | TATAGATCTCCCGCCGTCGCTCGCCGGCTCCAG | 75 | The coding region for the truncated LipB with deletion of N-terminal 70-amino acid residue. |
lipBER | CTTCTCGAGCTGCATGCTGCCGGCCCCGCG | ||
lipAEF | TATGGATCCGGCCGATGGCTACGCGGCGACGC | 73 | The coding region for the mature LipA |
lipAER | CTTAAGCTTTTACACGCCCGCCAGCTTCAG | ||
lipC21EF | CGCGGATCCGCTTCGCCCGGCCGCGTTCCC | 60 | The coding region for the mature LipC21 |
lipC21ER | CCCAAGCTTGCCGCGACACGGCCTGCTGCGC | ||
lipC24EF | CGCGGATCCGGCGCACCGGCCGTGTCCGA | 60 | The coding region for the mature LipC24 |
lipC24ER | CCCAAGCTTGGTGCAGCGTCTCGGGCGCGAG | ||
lipC24MF | GCTATGCAGGCGGCGCGATCGCGAC | 60 | pEDSF-lipB-lipC24-Ser 179 Ala |
lipC24MR | GCCGCCTGCATAGCCGATCATCGCG | ||
tmpF | CTTAGATCTCACGAACCCAGTTGACATAAG | 54 | Full length of the trimethoprim resistance gene |
tmpR | CTTAGATCTTTAGGCCACACGTTCAAG | ||
lipAIF | CTTGGATCCCGAGTATTGGTACGGCATCCAG | 53 | lipA gene fragment (named as lipA’) |
lipAIR | CTTCTCGAGTTACACGCCCGCCAGCTTCAGC | ||
gfpF-lipA | CTTCTGCAGATGGTGAGCAAGGGCGAGGA | 54 | Full length of the gfp gene (construction pBCMB-S3) |
gfpR-lipA | CTTCTGCAGTTACTTGTACAGCTCGTCCATG | ||
LipC24IF | TGCTCTAGAAATACGGGATGACCACGCTTGAT | 66 | LipC24 gene fragment (named as lipC24’) |
LipC24IR | CTTGGGCCCCGTTGAAACGGTCGTAGAGCCAC | ||
gfpF-lipC24 | GGAATTCCATATGATGGTGAGCAAGGGCGAGGA | 60 | Full length of the gfp gene (construction pBCMB-S5) |
gfpR-lipC24 | GGAATTCCATTTACTTGTACAGCTCGTCCATG |