Fig. 3From: Cell-bound lipases from Burkholderia sp. ZYB002: gene sequence analysis, expression, enzymatic characterization, and 3D structural modelSDS-PAGE analysis of LipC24 in different purification steps. M: protein marker; 1: the purified LipC24 by HiTrap DEAE FF anion-exchange chromatography column; 2: the purified LipC24 by HisTrap HP affinity chromatography column; 3: cell-free extract of E. coli Origami 2(DE3)-pEDSF-lipB-lipC24 Back to article page