Fig. 2

Map of the expression plasmid for lipA, lipC21 and lipC24. a pEDSF-lipB- lipA was derived from pACYCDuet-1, which was inserted lipA gene at the MCS1 site and the chaperone lipB gene at the MCS2 site; b pEDSF-lipC21 was derived from pET28a, which was inserted lipC21 gene at the MCS site. To obtain the soluble expression of lipC21, plasmid pEDSF-lipC21 and plasmid pGro7 must be co-transformed into E. coli BL21(DE3); c pEDSF-lipB-lipC24 was derived from pACYCDuet-1, which was inserted lipC24 gene at the MCS1 site and the chaperone lipB gene at the MCS2 site