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Fig. 3 | BMC Biotechnology

Fig. 3

From: Trigger factor assisted soluble expression of recombinant spike protein of porcine epidemic diarrhea virus in Escherichia coli

Fig. 3

Optimization of IPTG concentration (a), OD at induction (b) and harvest time (c) for soluble rGST-COE and rGST-S1D expression. The amount of soluble (S) and insoluble (I) fractions were compared relatively with each starting condition (IPTG = 0.1 mM, OD = 0.6, Time = 12 h), respectively, according to the band intensities of SDS-PAGE. The experiment was conducted twice independently, and the data from a representative experiment are shown

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BMC Biotechnology

ISSN: 1472-6750

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