Fig. 2

Effect of trigger factor (TF) on the solubility of rGST-COE (a) and rGST-S1D (b). TF was induced prior to the expression of recombinant proteins by adding L-arabinose (0.5 mg/ml) at 37 °C. Target proteins were induced by adding 0.1 mM IPTG at 15 °C for 24 h. Soluble (S) and insoluble (I) fractions were analyzed by SDS-PAGE. The protein bands for rGST-COE, rGST-S1D and TF are indicated. Lanes: −/−, no induction; A/-, induction with L-arabinose; −/I, induction with IPTG; A/I, induction with L-arabinose and IPTG