Skip to main content

Table 2 Effect of the arm length variation on knockin efficiency

From: Reliable handling of highly A/T-rich genomic DNA for efficient generation of knockin strains of Dictyostelium discoideum

Gene

Chromosome

Vector name

5′ arm length

3′ arm length

Total amount of knockin vector

aBsR clone

bPositive clone

Efficiency

carA-1

DDB_G0273397

2

pJ_cAR1_GFP

1.0 kb

1.0 kb

10 μg

0

-

0 %

     

20 μg

3

0/3

0 %

0/0

-

     

40 μg

>500

27/96

28 %

3/16

19 %

acaA

DDB_G0281545

3

pJ_acaA_GFP

0.6 kb

0.5 kb

40 μg

>200

5/11

46 %

3/8

38 %

regA

DDB_G0284331

4

pJ_regA_GFP_0.3

0.3 kb

1.0 kb

40 μg

124

0/96

0 %

0/28

0 %

  

pJ_regA_GFP_0.5

0.5 kb

1.0 kb

40 μg

138

0/96

0 %

0/48

0 %

  

pJ_regA_GFP_1.3

1.3 kb

1.0 kb

40 μg

180

56/96

58 %

9/16

56 %

dagA/crac

DDB_G0285161

4

pJ_crac_GFP_0.3

0.3 kb

0.6 kb

40 μg

>500

0/96

0 %

0/96

0 %

  

pJ_crac_GFP_1.9

1.9 kb

0.6 kb

40 μg

>500

10/16

63 %

8/51

16 %

erkB

DDB_G0283903

4

pJ_erkB_GFP_0.5/0.6

0.5 kb

0.6 kb

40 μg

>500

0/96

0 %

0/96

0 %

  

pJ_erkB_GFP_1.0/0.6

1.0 kb

0.6 kb

40 μg

>500

0/288c

0 %

0/96

0 %

  

pJ_erkB_GFP_1.0/1.7

1.0 kb

1.7 kb

40 μg

>500

16/30

53 %

4/16

25 %

  

pJ_erkB_GFP_0.5/1.7

0.5 kb

1.7 kb

40 μg

>500

14/30

47 %

5/16

31 %

  1. aTotal number of BsR clones from two experiments by using independently prepared materials
  2. bThe number of homologous recombinants obtained from two independent experiments was separately provided
  3. cOne clone with single cross-over at 5′ arm obtained from 288 of BsR colonies