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Table 2 Effect of the arm length variation on knockin efficiency

From: Reliable handling of highly A/T-rich genomic DNA for efficient generation of knockin strains of Dictyostelium discoideum

Gene Chromosome Vector name 5′ arm length 3′ arm length Total amount of knockin vector aBsR clone bPositive clone Efficiency
carA-1
DDB_G0273397
2 pJ_cAR1_GFP 1.0 kb 1.0 kb 10 μg 0 - 0 %
      20 μg 3 0/3 0 %
0/0 -
      40 μg >500 27/96 28 %
3/16 19 %
acaA
DDB_G0281545
3 pJ_acaA_GFP 0.6 kb 0.5 kb 40 μg >200 5/11 46 %
3/8 38 %
regA
DDB_G0284331
4 pJ_regA_GFP_0.3 0.3 kb 1.0 kb 40 μg 124 0/96 0 %
0/28 0 %
   pJ_regA_GFP_0.5 0.5 kb 1.0 kb 40 μg 138 0/96 0 %
0/48 0 %
   pJ_regA_GFP_1.3 1.3 kb 1.0 kb 40 μg 180 56/96 58 %
9/16 56 %
dagA/crac
DDB_G0285161
4 pJ_crac_GFP_0.3 0.3 kb 0.6 kb 40 μg >500 0/96 0 %
0/96 0 %
   pJ_crac_GFP_1.9 1.9 kb 0.6 kb 40 μg >500 10/16 63 %
8/51 16 %
erkB
DDB_G0283903
4 pJ_erkB_GFP_0.5/0.6 0.5 kb 0.6 kb 40 μg >500 0/96 0 %
0/96 0 %
   pJ_erkB_GFP_1.0/0.6 1.0 kb 0.6 kb 40 μg >500 0/288c 0 %
0/96 0 %
   pJ_erkB_GFP_1.0/1.7 1.0 kb 1.7 kb 40 μg >500 16/30 53 %
4/16 25 %
   pJ_erkB_GFP_0.5/1.7 0.5 kb 1.7 kb 40 μg >500 14/30 47 %
5/16 31 %
  1. aTotal number of BsR clones from two experiments by using independently prepared materials
  2. bThe number of homologous recombinants obtained from two independent experiments was separately provided
  3. cOne clone with single cross-over at 5′ arm obtained from 288 of BsR colonies