Table 1 Efficiency of knockin vector construction by using a linear DNA cloning system
Arm length | A/T-content | |||||||
|---|---|---|---|---|---|---|---|---|
Gene | Vector name | 5′ arn | 3′ arm | 5′ arm | 3′ arm | Knockin cassette Total | Positive clone | Efficiency |
carA-1 DDB_G0273397 | pJ_carA1_GFP | 1.0 kb | 1.0 kb | 72 % | 85 % | 75 % (4.6 kb) | 12/16 | 75 % |
acaA DDB_G0281545 | pJ_acaA_GFP | 0.6 kb | 0.5 kb | 74 % | 83 % | 72 % (3.7 kb) | 8/8 | 100 % |
regA DDB_G0284331 | pJ_regA_GFP_0.3 | 0.3 kb | 1.0 kb | 69 % | 79 % | 73 % (3.8 kb) | 8/8 | 100 % |
pJ_regA_GFP_0.5 | 0.5 kb | 1.0 kb | 69 % | 79 % | 73 % (4.0 kb) | 7/8 | 88 % | |
pJ_regA_GFP_1.3 | 1.3 kb | 1.0 kb | 76 % | 79 % | 74 % (4.8 kb) | 7/8 | 88 % | |
dagA/crac DDB_G0285161 | pJ_crac_GFP_0.3 | 0.3 kb | 0.6 kb | 69 % | 80 % | 72 % (3.7 kb) | 8/8 | 100 % |
pJ_crac_GFP_1.9 | 1.9 kb | 0.6 kb | 70 % | 80 % | 72 % (5.0 kb) | 11/16 | 69 % | |
erkB DDB_G0283903 | pJ_erkB_GFP_0.5/0.6 | 0.5 kb | 0.6 kb | 64 % | 66 % | 70 % (3.6 kb) | 8/8 | 100 % |
pJ_erkB_GFP_1.0/0.6 | 1.0 kb | 0.6 kb | 72 % | 66 % | 71 % (4.1 kb) | 7/8 | 88 % | |
pJ_erkB_GFP_1.0/1.7 | 1.0 kb | 1.7 kb | 72 % | 77 % | 74 % (5.2 kb) | 4/8 | 50 % | |
pJ_erkB_GFP_0.5/1.7 | 0.5 kb | 1.7 kb | 64 % | 77 % | 73 % (4.7 kb) | 4/8 | 50 % | |