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Fig. 3 | BMC Biotechnology

Fig. 3

From: Application of surface plasmon resonance imaging to monitoring G protein-coupled receptor signaling and its modulation in a heterologous expression system

Fig. 3

R P/S increase is due to PKC translocation. a and b The effect of TPA (100 nM) was examined in non-transfected HEK293T cells pretreated with saline containing the PKC inhibitor BIM1 (300 nM) or the control vehicle alone. BIM1 continued to be present in the saline throughout the measurement. The data were obtained from cultures different from those in Fig. 2. a Sample images of the reflection of the P-polarized light. b Mean time-plots of R P/S . Error bars, ±SEM. ***, p < 0.0001 at 25 min of the TPA application onset (van der Waerden rank sum test)

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BMC Biotechnology

ISSN: 1472-6750

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