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Fig. 1 | BMC Biotechnology

Fig. 1

From: Application of surface plasmon resonance imaging to monitoring G protein-coupled receptor signaling and its modulation in a heterologous expression system

Fig. 1

Experimental set-up. a HEK293 cells were cultured on a 50 nm-thick gold-plated slide glass. The cell chamber was surrounded with a silicone rubber wall attached to the slide glass. b Configuration of SPR imaging. SPR takes place, consuming the energy of the evanescent light derived from the P-polarized component of the incident light. PKC accumulated on the plasma membrane alters the mode of SPR, and this results in changes in the consumption of the evanescent light energy and the intensity of the reflection of the P-polarized light. Theta, angle of incidence. c Sample plots of R P/S against the angle of incidence (average of 5 ROIs) before and after a 15-min application of the potent PKC activator TPA (100 nM)

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BMC Biotechnology

ISSN: 1472-6750

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