TY - JOUR AU - Nonobe, Yuki AU - Yokoyama, Tomoki AU - Kamikubo, Yuji AU - Yoshida, Sho AU - Hisajima, Nozomi AU - Shinohara, Hiroaki AU - Shiraishi, Yuki AU - Sakurai, Takashi AU - Tabata, Toshihide PY - 2016 DA - 2016/04/12 TI - Application of surface plasmon resonance imaging to monitoring G protein-coupled receptor signaling and its modulation in a heterologous expression system JO - BMC Biotechnology SP - 36 VL - 16 IS - 1 AB - G protein-coupled receptors (GPCRs) are ubiquitous surface proteins mediating various biological responses and thus, important targets for therapeutic drugs. GPCRs individually produce their own signaling as well as modulate the signaling of other GPCRs. Real-time observation of GPCR signaling and modulation in living cells is key to molecular study of biological responses and pharmaceutical development. However, fluorescence imaging, the technique widely used for this purpose, requires a fluorescent dye which may inhibit biological responses or a fluorescent-tagged target protein created through time-consuming genetic manipulation. In this study, we applied two-dimensional surface plasmon resonance (SPR) imaging to monitoring the translocation of protein kinase C (PKC), a major GPCR-coupled signaling molecule in the widely used HEK293 cell lines and examined whether the signaling of, and, modulation between heterologously expressed GPCRs can be measured without fluorescent labeling. SN - 1472-6750 UR - https://doi.org/10.1186/s12896-016-0266-9 DO - 10.1186/s12896-016-0266-9 ID - Nonobe2016 ER -