Fig. 5
Kinetic analysis of human renin with recombinant oANGEcoli and oANGCHO. Renin activity in the presence of oANGEcoli (solid line) and oANGCHO (dotted line) was analyzed by steady-state enzyme kinetics. Various concentrations of oANG were incubated with human renin (25 pM) at 37 °C in sodium phosphate buffer (pH 7.2) containing DFP and EDTA. The amount of AI produced was measured by enzyme-linked immunosorbent assay (ELISA). The error bars represent the standard deviation of the velocities (N = 3)