Fig. 4
Protein structure and stability analysis of recombinant oANGEcoli and oANGCHO. Purified oANGEcoli (solid line) and oANGCHO (dotted line) were analyzed by CD (a) and DSF (b). CD data were measured at 25 °C in a pH 7.4 buffer solution containing 0.5 mg/mL oANG, 20 mM sodium phosphate, and 20 mM (oANGEcoli) or 33 mM (oANGCHO) KCl. DSF data were measured with 10 μM oANG in 5× SYPRO Orange, 200 mM HEPES, and 0.15 M KCl (pH 7.5). Five typical plots for each preparation are shown. c Comparison of the T m of oANGEcoli and oANGCHO. T m values are plotted as open circles. The horizontal line and error bars are the mean value and standard deviation of the T m values, respectively (oANGEcoli, N = 30; oANGCHO, N = 31). *P < 0.0001 by Student’s t-test