Fig. 3
Protein size analysis of recombinant oANGEcoli and oANGCHO. The sizes of oANGEcoli (solid line) and oANGCHO (dotted line) were determined by analytical size-exclusion gel filtration (a) and DLS (b). Purified oANG was loaded onto a Superdex 200 10/300 GL column equilibrated with 2 mM HEPES and 0.20 M KCl (pH 8.0). The elution volumes of the marker proteins are shown. The calibration curve is shown in the inset. DLS data were measured at 25 °C in a pH 7.4 buffer solution containing 0.5 mg/mL oANG, 20 mM sodium phosphate, and 20 mM (oANGEcoli) or 33 mM (oANGCHO) KCl. The size distribution by percent volume is presented