Skip to main content
Fig. 2 | BMC Biotechnology

Fig. 2

From: Escherichia coli-based production of recombinant ovine angiotensinogen and its characterization as a renin substrate

Fig. 2

SDS-PAGE analysis of recombinant oANG expressed from a tac promoter. a Comparison of recombinant oANG expressed from either a T7 promoter or a tac promoter. Recombinant oANG expression was induced with IPTG (0.1 mM) at 37 °C. E. coli cells transformed with either pET-11a-oANG (T7 promoter) or pTAC-oANG-His (tac promoter) were diluted to a similar OD600 value and disrupted by sonication. Recombinant oANG in the soluble (S) and insoluble (I) fractions was visualized by western blotting. Lane C, control preparation of oANG produced by pTAC-oANG-His. b SDS-PAGE analysis of the purified recombinant oANG preparations. The pooled fractions after each purification step were separated by SDS-PAGE and stained with CBB. Lane M, molecular weight marker; lane 1, cell lysate; lane 2, supernatant of cell lysate; lane 3, precipitant of cell lysate; lane 4, pooled fractions after Ni-NTA Superflow Cartridge column chromatography; lane 5, after HiTrapQ HP column chromatography; lane 6, after HiLoad 16/60 Superdex 200 pg column chromatography. Molecular weights of the marker proteins are shown on the left. Arrowhead on the right shows the size of oANG

Back to article page