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Fig. 1 | BMC Biotechnology

Fig. 1

From: Escherichia coli-based production of recombinant ovine angiotensinogen and its characterization as a renin substrate

Fig. 1

Expression screening of recombinant oANG in E. coli. Comparison of oANG expression and solubility using different E. coli (DE3) host strains and IPTG concentrations at 37 °C (a) and 25 °C (b). Recombinant oANG was expressed from pET-11a-oANG in E. coli (DE3) lysogens following induction with IPTG (0.1 and 1.0 mM), separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), and then stained with Coomassie Brilliant Blue (CBB). Lane M, molecular marker; lane T, total cell lysate; lane S, soluble fraction; lane I, insoluble fraction. Western blot analysis of oANG expressed at 37 °C (c) and 25 °C (d) induced with IPTG (0.1 mM). Soluble (S) and insoluble (I) fractions of the cell lysates were analyzed by western blotting with an anti-oANG polyclonal antibody. Molecular markers (M) were visualized by CBB staining. The molecular weights of the marker proteins are shown on the left. The arrowhead on the right shows the size of oANG

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