Fig. 3From: Towards next generation maggot debridement therapy: transgenic Lucilia sericata larvae that produce and secrete a human growth factortTA-mediated pdgf-b expression in transgenic L. sericata. a Schematic of the DR4 tTA driver and EF-PDGF tTA-regulated effector gene constructs in piggyBac transformation vectors. b Genomic DNA sequence adjacent to 3' pBac for each strain. c DR4#14, EF-PDGF#11, and DR4#14 + EF-PDGF#11 larvae under white light. d Relative expression of pdgf-b mRNA in control effector alone and tTA-driver plus effector larvae. qRT-PCR analysis was performed on RNA isolated from whole larvae and normalized to the 28 s rRNA reference geneBack to article page