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Fig. 1 | BMC Biotechnology

Fig. 1

From: Towards next generation maggot debridement therapy: transgenic Lucilia sericata larvae that produce and secrete a human growth factor

Fig. 1

Heat inducible expression of pdgf-b mRNA in transgenic PD-1‚ÄČL. sericata. a Schematic of heat-inducible pdgf-b gene construct in a piggyBac transformation vector with a ZsGreen marker gene. b Genomic DNA sequence adjacent to the 5' pBac end in the PD-1 transgenic line. The TTAA insertion site is underlined. c RT-PCR amplification of pdgf-b on total RNA obtained from first instar PD-1 larvae that had been given a heat shock (+HS) or no heat shock (-HS)

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