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Fig. 3 | BMC Biotechnology

Fig. 3

From: Depleting extracellular vesicles from fetal bovine serum alters proliferation and differentiation of skeletal muscle cells in vitro

Fig. 3

Removing FBS-derived vesicles from bovine serum affects gene expression. Quantitative RT-PCR of relevant genes for proliferation (Cyclin D1, Sirt1), differentiation (MyoD, MyoG), metabolism (Glut4) and vesicle trafficking and EV release (TSG101, VPS37B, VPS4). Data are expressed as fold compared to CM, normalized by house keeping gene (HKG). In white, cells grown in control media containing FBS-EVs (CM), in black, cells grown in CM depleted of FBS-EVs (SEDM). a C2C12 myoblasts; b L6 rat myoblasts; c human primary myoblasts

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