Table 3 Strains and plasmids used in this study
From: Metabolic engineering of Escherichia coli for the production of hydroxy fatty acids from glucose
Strains or plasmids | Genotype/Description | Sources |
|---|---|---|
Strains | ||
E. coli BL21 star(DE3) | F − ompT hsdS B (rB − mB −) gal dcm rne131 (DE3) | Invitrogen |
E. coli BL21 star(DE3) ΔfadD | Knockout of fadD encoding acyl-CoA synthetase | [42] |
Plasmids | ||
pET28a(+) | Kanr oripBR322 lacI q T7p | Novagen |
pET30a(+) | Kanr oripBR322 lacI q T7p | Novagen |
pACYCDuet-1 | Cm r oriP15A lacI q T7p | Novagen |
pE-‘tesA | pET30a(+) harboring E. coli ‘tesA | This study |
pE-A1 | pET28a(+) harboring B. megaterium CYP102A1 | This study |
pE-A1’tesA | pET28a(+) harboring both E. coli ‘tesA and B. megaterium CYP102A1 | This study |
pA-acc | pACYCDuet-1 harboring E. coli ACCase | [43] |