Skip to main content

Table 3 Strains and plasmids used in this study

From: Metabolic engineering of Escherichia coli for the production of hydroxy fatty acids from glucose

Strains or plasmids Genotype/Description Sources
Strains
E. coli BL21 star(DE3) F ompT hsdS B (rB mB ) gal dcm rne131 (DE3) Invitrogen
E. coli BL21 star(DE3) ΔfadD Knockout of fadD encoding acyl-CoA synthetase [42]
Plasmids
pET28a(+) Kanr oripBR322 lacI q T7p Novagen
pET30a(+) Kanr oripBR322 lacI q T7p Novagen
pACYCDuet-1 Cm r oriP15A lacI q T7p Novagen
pE-‘tesA pET30a(+) harboring E. coli ‘tesA This study
pE-A1 pET28a(+) harboring B. megaterium CYP102A1 This study
pE-A1’tesA pET28a(+) harboring both E. coli ‘tesA and B. megaterium CYP102A1 This study
pA-acc pACYCDuet-1 harboring E. coli ACCase [43]