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Table 1 Primers used for qPCR studies

From: A simple, accurate and universal method for quantification of PCR

Gene Accession number Primer sequence Annealing temperature Amplicon size
Lambda NC_001416 For: CGGCGTCAAAAAGAACTTCC 60 °C 92 bp
Rev: GCATCCTGAATGCAGCCATA
Lambda NC_001416 For: CGGCGTCAAAAAGAACTTCC 60 °C 501 bp
Rev: TGATCCCACCTCATTTTCATGT
Ar NM_013476.3 For: ACCCAAAACCCACCTTGTT 64 °C 214 bp
Rev: ACGCAGCAGATTCAAAATGT
Actb NM_007393.3 For: AGCCATGTACGTAGCCATCC 64 °C 377 bp
Rev: GGAACCGCTCGTTGCCAATA
Rpl19 NM_009078.2 For: GATCATCCGCAAGCCTGTGACT 60 °C 362 bp
Rev: GTGCTTCCTTGGTCTTAGAC
Hmbs NM_001110251.1 For: TGATGAAAGATGGGCAACTG 64 °C 160 bp
Rev: ATGTTACGGGCAGTGATTCC
Rps9 NM_029767.2 For: ATTACATCCTGGGGCCTGAAG 64 °C 210 bp
Rev: AAGGAGAACGGAGGGAGAAG
Ppia BC083076.1 For: ATCACGGCCGATGACGAGCC 64 °C 217 bp
Rev: TCTCTCCGTAGATGGACCTGC
Hprt NM_013556.2 For: GATACAGGCCAGACTTTGTTGG 64 °C 154 bp
Rev: AACTTGCGCTCATCTTAGGC
CD40 NG_007279 For: GAAACTGGTGAGTGACTGC 60 °C 341 bp
Rev: CACATTGGAGAAGAAGCC
IL7R NG_009567 For: CTGGAACATCTTTGTAAGAAACCAAG 60 °C 127 bp
Rev: TAGCTTGAATGTCATCCACCCT
GAPDH NG_007073.2 For: TCCACCACCCTGTTGCTGTA 60 °C 231 bp
Rev: ACCACAGTCCATGCCATCAC