Table 2 Conversion to fGly is highly dependent on cell culture conditions
Media | Culture day | Density (million/mL) | Viability | Titer (mg/L) | Conversion |
|---|---|---|---|---|---|
PF-CHO | Day 7 | 2.8 | 90 % | 29.4 | 98 % |
Day 10 | 4.5 | 95 % | 49.7 | 96 % | |
Day 14 | 3.4 | 80 % | 101.7 | 86 % | |
FortiCHO | Day 5 | >10 | 99 % | 4.4 | 70 % |
Day 7 | 17 | 99 % | 12.6 | 42 % | |
Day 10 | 10 | 80 % | 33.2 | 36 % | |
Day 11 | 5.3 | 66 % | 48.7 | 20 % |