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Table 2 Conversion to fGly is highly dependent on cell culture conditions

From: Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementing culture media with copper(II)

Media Culture day Density (million/mL) Viability Titer (mg/L) Conversion
PF-CHO Day 7 2.8 90 % 29.4 98 %
Day 10 4.5 95 % 49.7 96 %
Day 14 3.4 80 % 101.7 86 %
FortiCHO Day 5 >10 99 % 4.4 70 %
Day 7 17 99 % 12.6 42 %
Day 10 10 80 % 33.2 36 %
Day 11 5.3 66 % 48.7 20 %