Skip to main content

Advertisement

Table 1 FGE form does not correlate with conversion yield

From: Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementing culture media with copper(II)

    Transient Ab 1, CH-CT Transient Ab 2, Hinge Stable Ab 1, CH-CT Stable Ab 2, CH-CT Stable Ab 2, Hinge
Clone FGE forma FGE levels Titers (mg/L) Conversion Titers (mg/L) Conversion Titers (mg/L) Conversion Titers (mg/L) Conversion Titers (mg/L) Conversion
395-12 WT Low 11.8 96 % 10.5 81 % ND ND 2.1 99 % 1.6 99 %
395-12 WT Low 3.7 90 % 6.6 69 % ND ND 1.6 98 % ND ND
408-102 ∆Fur + KDEL High 11.5 95 % 9.9 81 % ND ND 1.1 98 % 1.2 90 %
409-107 ∆Fur + KDEL Low 1.4 83 % 6.8 69 % 2 97 % 1.1 97 % ND ND
407-118 +KDEL High 11.8 97 % ND ND 4.7 97 % 0.9 98 % 1.3 99 %
406-127 ∆Fur Med 1.9 80 % 7.4 54 % 7.8 92 % 1.5 94 % ND ND
409-35 ∆Fur + KDEL Med ND ND 6.5 68 % 1.7 92 % 2 98 % ND ND
  1. a∆Fur, deleted furin cleavage site