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Fig. 7 | BMC Biotechnology

Fig. 7

From: Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementing culture media with copper(II)

Fig. 7

A GPEx® clonal cell line expressing CT-tagged antibody afforded high fGly conversion and high titers in a 100 L bioreactor production run. Cells were cultured in HyCell™ media supplemented with 50 μM copper(II) sulfate. The protein concentration and integral of viable cell density is shown. Cultures were terminated when viabilities were ≤ 80 %. Titers of 2.9 g/L were reached. The cells demonstrated a specific productivity of 43 pg/cell/d, and the resulting antibody had fGly conversion yields of 96.4 %

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