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Fig. 2 | BMC Biotechnology

Fig. 2

From: Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementing culture media with copper(II)

Fig. 2

fGly conversion is affected by cell culture media and time in culture. A CHO-S clone (clone 101) stably expressing FGE + KDEL and CT-tagged antibody was cultured in PF-CHO™ or FortiCHO™, fed with 10 % Efficient Feed C on Days 3, 5, and 7, and supplemented with 3 g/L glucose when levels dropped below 3 g/L. Samples of media were taken at the indicated days (harvest was at day 11 and day 14 for FortiCHO™ and PF-CHO™ cultures, respectively). Titers (indicated by the numbers atop bars, mg/L) were assessed by ELISA, and conversion was determined by mass spectrometry. Representative data are shown (n =2 experiments)

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