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Fig. 2 | BMC Biotechnology

Fig. 2

From: Production of antigen-specific human IgGs by in vitro immunization

Fig. 2

a The covalent coupling of Tat101 to ZZ is absolutely required to trigger an anti-Tat IgG Ab response. PBMCs were incubated with or without anti-CD40/IL-4/IL-21, ZZTat101, ZZTat101 + anti-CD40/IL-4/IL-21, ZZ + anti-CD40/IL-4/IL-21, ZZ + Tat101 + anti-CD40/IL-4/IL-21, Tat101 + anti-CD40/IL-4/IL-21, respectively. Then, supernatants were collected and added to Tat101-coated plates. Presence of anti-Tat IgG was assessed as in Fig. 1a. b The anti-Tat IgG response requires preservation of the Tat lysines. PBMCs were incubated with or without anti-CD40/IL-4/IL-21, ZZTat101 + anti-CD40/IL-4/IL-21, ZZTat101ϕ + anti-CD40/IL-4/IL-21, respectively. Presence of anti-Tat IgG was assessed in supernatants as in Fig. 1a (*: p < 0.05)

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