Fig. 1
From: Production of antigen-specific human IgGs by in vitro immunization
a An anti-Tat IgG Ab response is raised in vitro when PBMCs are incubated with ZZTat101 and a cytokine/activator cocktail. PBMCs (5 × 105 cells per well) were incubated with or without CD40L/IL-4/IL-21, anti-CD40/IL-4/IL-21, ZZTat101, ZZTat101 + CD40L/IL-4/IL-21, ZZTat101 + anti-CD40/IL-4/IL-21, respectively. Then, supernatants were collected and added to Tat101-coated plates. After overnight incubation, a peroxidase-conjugated anti-human IgG was added and enzymatic activity was determined using ABTS as substrate. b The anti-Tat IgG Ab response is specific to Tat101. Supernatants from PBMCs incubated with ZZTat101 + anti-CD40/IL-4/IL-21 were mixed with either a dilution buffer or a fixed amount (10 μg/mL) of three different Ags (Tat101, ovalbumin, lysozyme) and incubated in Tat101-coated plates. Presence of anti-Tat IgG was assessed as in A (**: p < 0.01; ***: p < 0.001)