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Table 1 Oligonucleotide primers used in this study

From: Successful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115

Name

Sequence

A-up

5‘-GGAATTCCAT ATGGGGCAATCACACACAC-3‘

P-down

5‘-CCGGAATTCTCAAGCCATTGCGGCAACGA-3‘

P-down(his)

5‘-CCGGAATTCTCAGTGATGATGATGATGATGAGCCATTGCGGCAACGA-3‘

B-down(his)

5‘-TGGCTGCTGCCCATATCTATATCTCCTTTCACGCTGGCTCCAGGTAGTCATC-3‘

P-up(his)

5‘-ATATGGGCAGCAGCCATCATCATCATCATCACAAAGACGAACGGTTTCCATT-3‘

B-down(gst)

5‘-CTATATCTCCTTTCACGCTGGCTCCAGGTAGTCATC-3‘

P-up(gst)

5‘-GAAAGGAGATATAGATATGTCCCCTATACTAGGTTA-3‘

A*-up

5‘-TCTTCGTCTGCACCCTGTGCGCGTGCTACCCATGGCC-3‘

A*-down

5‘-TGACGGCCATGGGTAGCACGCGCACAGGGTGCAGAC-3‘

  1. The underlined code represents the gene start code or the Ser115 to Ala115 mutation. Italicized letters denote the NdeI and EcoRI recognition sites, respectively