Skip to main content

Table 1 Oligonucleotide primers used in this study

From: Successful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115

Name Sequence
A-up 5‘-GGAATTCCAT ATGGGGCAATCACACACAC-3‘
P-down 5‘-CCGGAATTCTCAAGCCATTGCGGCAACGA-3‘
P-down(his) 5‘-CCGGAATTCTCAGTGATGATGATGATGATGAGCCATTGCGGCAACGA-3‘
B-down(his) 5‘-TGGCTGCTGCCCATATCTATATCTCCTTTCACGCTGGCTCCAGGTAGTCATC-3‘
P-up(his) 5‘-ATATGGGCAGCAGCCATCATCATCATCATCACAAAGACGAACGGTTTCCATT-3‘
B-down(gst) 5‘-CTATATCTCCTTTCACGCTGGCTCCAGGTAGTCATC-3‘
P-up(gst) 5‘-GAAAGGAGATATAGATATGTCCCCTATACTAGGTTA-3‘
A*-up 5‘-TCTTCGTCTGCACCCTGTGCGCGTGCTACCCATGGCC-3‘
A*-down 5‘-TGACGGCCATGGGTAGCACGCGCACAGGGTGCAGAC-3‘
  1. The underlined code represents the gene start code or the Ser115 to Ala115 mutation. Italicized letters denote the NdeI and EcoRI recognition sites, respectively