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Fig. 5 | BMC Biotechnology

Fig. 5

From: Successful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115

Fig. 5

Energetic calculation of the proposed proton transfer pathway. a The structure of the proposed NHase catalytic model in the active center. The big red ball represents the cobalt ion. The Ser115 was shown by black dashed arrow. The H1 represents the proton of substrate transition state. The H2 represents the proton of Ser115(OH). The type I is the proton transfer pathway: H1(substrate) → H2(Ser115 of NHase) → N(substrate). The type II (direct transfer) is the proton transfer pathway: H1(substrate) → N(substrate). b Energy calculation of proposed transfer pathway (type I): the proton transfer pathway via the proton of OH(Ser115) (red dashed line). c Energy calculation of proposed transfer pathway (type II): the proton direct transfer pathway (not via the proton of OH(Ser115)) (red dashed line)

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