Fig. 3

Expression and purification of recombinant NHases. a 1, mark; 2, control; 3, cell extracts (pET-ABP); 4, purified NHase (pET-ABP); 5, cell extracts (pET-AB(his-P)); 6, purified NHase (pET-AB(his-P)); 7, cell extracts (pET-AB(P-his)); 8, purified NHase (pET-AB(P-his)). b Molecular mass determination on a Superdex 200 prep grade column. Standard proteins are as follows: (i) thyroglobulin (bovine thyroid) (669 kDa); (ii) ferritin (horse spleen) (440 kDa); (iii) aldolase (rabbit muscle) (158 kDa); (iv) conalbumin (chicken egg white) (75 kDa); and (v) ovalbumin (hen egg) (43 kDa). The open circle represents the determined molecular mass. The values represent the means ± SD for at least three independent experiments [5]