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Fig. 1 | BMC Biotechnology

Fig. 1

From: Successful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115

Fig. 1

The catalytic pathway involving Ser115 during NHase maturation. a First step: atoms of O(CEA)-H(H2O) and OH(H2O)-Co3+ participates in NHase activation (dashed line). Second step: the proton of OH(Ser115) participates in the proton transfer pathway (dashed line). b The oxygen of the phenolate of the conserved Tyr68 was ionized by the general base Ser115 during NHase activation. c The oxygen of the phenolate of the conserved Tyr68 was ionized by the general base Ser115 with the help of Tyr72 during NHase activation

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BMC Biotechnology

ISSN: 1472-6750

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