Fig. 4

Poly (I:C)-induced cell death is reduced in cells with impaired TLR3, IRF3 and p65 expression. 24 h after transfection of 2 μg/ml Poly (I:C) with DOTAP, fractional cell death was measured by flow cytometry using propidium iodide. We compared wt Hela cells and polyclonal HeLa cell lines generated with the nine different gRNAs targeting TLR3, IRF3 and p65 genes and the control gRNA targeting GFP. TLR3 gRNA-1 cells denoted with a star (*) were not treated with IZsCD95L, while ctr. denotes HeLa wt cells transfected with a non-triphosphate 19-mer RNA control to account for cell death due to transfection with DOTAP. Shown are means ± s.e.m. of 3 independent experiments