Fig. 2

Death ligands induce cell death in various cell types within a few hours. Cell death was measured by observing cell morphology by microscopy. a Cell death kinetics of A549, HepG2 and HeLa cells using IZsTRAIL and IZsCD95L, with and without co-treatment with 5 to 50 μg/ml cycloheximide (CHX). b Cell death of LN-18, HT-1080, MDA-MB-231 and MCF10A cells treated for 5 h with IZsCD95L (denoted CD95L) or IZsTRAIL (denoted TRAIL), with or without 5 μg/ml CHX. c HeLa cell death kinetics comparison between IZsCD95L and puromycin. While cell death occurred after 2 h with IZsCD95L, it saturated at 30 h with puromycin. b and c: mean ± s.d. of 3 different fields of view with at least 50 cells each