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Fig. 6 | BMC Biotechnology

Fig. 6

From: Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen

Fig. 6

Binding analysis of recombinant SNAP-TTC-BG647 on polyclonally activated CD19+ B cells. CD19+ B cells were isolated from leukocyte filters by density gradient centrifugation and magnetic cell separation with anti-CD19 beads. Isolated B cells were activated by adding 2.5 μg/ml CpG and 50 ng/ml IL-21 to the cell culture medium. On day 4 of cultivation, the cells were analyzed by flow cytometry. Surface a and intracellular b staining with the recombinant SNAP-TTC-BG 647 (1:25) was performed

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BMC Biotechnology

ISSN: 1472-6750

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