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Fig. 3 | BMC Biotechnology

Fig. 3

From: Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen

Fig. 3

Binding analysis of recombinant TTC-based proteins to TTC-reactive hybridoma cells. Equimolar amounts (100 nM) of TTC (c) and TTC-ETA’ (d) were used for binding analysis to the TTC-reactive hybridoma cell line 5E4 (a) compared to the control hybridoma cell line 8.18-C5 (b). Detection of bound proteins was carried out using an Alexa Fluor® 488-coupled anti-His5 antibody. Staining with Alexa Fluor 488-coupled anti-His5 antibody (b) and unstained cells (a) served as controls. Binding analysis of 100 nM SNAP-TTC coupled to the SNAP-Surface® 647 fluorescence dye (b) to 5E4 hybridoma cells (c) and to the control hybridoma cell line 8.18-C5 (d). Unstained cells served as control (a)

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BMC Biotechnology

ISSN: 1472-6750

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