Fig. 4
Detection of mASAL in transgenic rice. a Western blot analysis of total protein extracts isolated from the leaves of 35SmASAL, six independent T0 transgenic rice lines (RSR4, RSR7, RSR20, RSR28, RSR34, and RSR45 in lane 3–8) and b six T1 progeny plants of RSR20 (T12, T14), RSR28 (T11, T13) and RSR34 (T18,T19) (lane 1–8). Lane 1, purified mASAL; lane 2, protein extract from untransformed control plant. c ELISA analysis for expression of mASAL in total soluble protein in six T0 transformants (RSR4, RSR7, RSR20, RSR28, RSR34 and RSR45) and d in six T1 progeny plants of line RSR20 (T12, T14), RSR28 (T11, T13), and RSR34 (T18, T19). The bars represent the mean ELISA reading of three replicas per sample of three experiments