Fig. 3
Southern blot analysis of the PCR positive T1 and T2 transformants. a Southern blot analysis of HindIII digested genomic DNA from leaves of six individual T1 progeny plants of corresponding T0 lines [RSR4 (T13), RSR7 (T14), RSR20 (T12), RSR28 (T11), RSR34 (T18) and RSR45(T16)] in lanes 3–8, respectively; lane 8, RSR45(T16), a segregating progeny showing absence of mASAL gene cassette. b Southern blot analysis of HindIII digested genomic DNA from leaves of four T2 progenies of RSR7 (T14) plant in lanes 3–6, respectively. mASAL gene was used as positive control (lane 1) and HindIII digested genomic DNA from control plants as negative control (lane 2). Approximate DNA molecular weight markers are indicated on the left