Fig. 4

Thermal stability of NitraS-ATII. The enzymes were incubated at high temperatures for 30 s (a) or 1 min (b) prior to performing the reaction and measuring the residual activity. Two-way ANOVA test followed by Bonferroni post-hoc test was performed using GraphPad Prism®(Windows® version 5.00). *** and ** indicate p-values lower than 0.001 and 0.01, respectively