Fig. 3

Biochemical characterization of NitraS-ATII. a. Effect of pH on nitrilase activity was assessed in acetate buffer (pH 3.5–5.0), phosphate buffer (pH 6.0–8.0) and carbonate buffer (pH 9.0–11.0). Optimum activity was achieved at pH 7.0. b. Effect of temperature on nitrilase activity. Optimum temperature was determined to be 40 °C and activity was nearly abolished at higher temperatures. c. Residual nitrilase activity after incubation at different temperatures for different periods. d. Nitrilase activity at different NaCl concentrations. Decrease in activity was observed with increase in salt concentration. All assays used 400 mmol.L^-1 succinonitrile as substrate