Fig. 1

The NitraS-ATII protein. a Amino acid sequence of the native NitraS-ATII with the catalytic triad of glutamate (D47), lysine (K129) and cysteine (C163) shown in red. b Three dimensional model of NitraS-ATII protein. The model was obtained using Phyre2 tool with 100 % confidence and visualized using pyMOL. Secondary structures were colored as follows: α-helices in red, β-sheets in yellow and loops in green. Residues of the catalytic triad are shown as stick representation, with carbon atoms in cyan, nitrogen atoms in red, oxygen atoms in blue and sulfur atoms in yellow. c Superimposition of three-dimensional structure models of NitraS-ATII and nitrilase from Rhodobacter sphaeroides LHS-305. NitraS-ATII, colored according to secondary structure, was superimposed onto R. sphaeroides LHS-305 nitrilase, shown in blue. Residues of the catalytic triad showed perfect superimposition