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Fig. 5 | BMC Biotechnology

Fig. 5

From: Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III in Pichia pastoris

Fig. 5

Evaluation of Immunogenicity of purified recombinant EDIII. a Coomassie stained 15 % SDS-PAGE showing purified recombinant EDIII as a ∼ 14 kDa band in five separate elution fractions (E1 to E5) after Ni-NTA chromatography. b Determination of anti-DENV serotype-3 neutralizing antibody titers using flow-cytometry based neutralization test. Mouse anti-EDIII pooled antisera (blue) and mock pooled antisera (red) were tested at various dilutions (X-axis) and percent viral infection (Y-axis) was calculated. The dotted line represents 50 % infection. The error bars represent standard deviation of two technical replicate

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BMC Biotechnology

ISSN: 1472-6750

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