Skip to main content
Fig. 4 | BMC Biotechnology

Fig. 4

From: Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III in Pichia pastoris

Fig. 4

Determination of intracellularly retained EDIII. Western blot of induced P. pastoris cell lysate [day 4 harvest with different CA concentration 0.0 to 2.0 % (w/v) supplemented BMM media] to determine intracellular EDIII protein at two induction temperatures. a 20 °C induced cell lysate showing presence of ~14 kDa EDIII protein band upto 0.5 % CA (w/v) whereas, b 30 °C induced showing gradual decrease in intracellular EDIII protein from 0 to 1.5 % CA (w/v). Anti-EDIII 24A12 mAb & goat anti-mouse HRP were used as primary & secondary antibody respectively. N represents negative control (KM71H cells transformed with pPICZαA) and P represents positive control (purified EDIII). Similar trends were observed with the cell mass (intracellular EDIII) obtained from two independent inductions (Additional file 9)

Back to article page