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Table 1 Assaying integrase mediated site-specific recombination in yeast S.cerevisiae

From: Comparison and optimization of ten phage encoded serine integrases for genome engineering in Saccharomyces cerevisiae

Integrase PCR for site specific recombination attL and attR PCR Sequence for attL (correct sample /checking number) Sequence for attR (correct sample/checking number)
ΦBT1 9/10+; 10/10+; 10/10; 10/10 15/15 15/15
10/10+; 10/10+; 10/10; 10/10 15/15 15/15
TP901 10/10+; 10/10+; 10/10; 10/10 15/15 15/15
TG1 9/10+; 9/10+; 9/10; 9/10 15/15 15/15
SPBC 10/10+; 10/10+; 10/10; 10/10 15/15 15/15
R4 9/10+; 8/10+; 8/10; 9/10 15/15 15/15
ΦC31 10/10+; 10/10+; 10/10; 10/10 15/15 15/15
BxB1 10/10+; 10/10+; 10/10; 10/10 15/15 15/15
RV 2/10+; 2/10+; 22/95; 2/10 15/15 15/15
MR11 9/10+; 10/10+; 10/10; 10/10 15/15 15/15
Φ370 8/10+; 7/10+; 9/10; 9/10 15/15 15/15
A118 6/10+; 5/10+; 7/10; 7/10 15/15 15/15
ΦC1 0/50+; 0/50+; 0/50; 0/50 NA NA
ΦK38 9/10+; 8/10+; 10/10; 9/10 15/15 15/15
  1. Table 1 PCR was carried out following transformation to measure the proportion of Leu+ clones that had undergone recombinase mediated cassette exchange. At least 10 clones were analysed from the strains containing the nls + integrase or the nls- integrase. (+ or – superscript respectively). Fifteen PCR products derived from each type of strain were confirmed by DNA sequencing as corresponding to reciprocal and conservative site-specific recombinants. In the case of RV integrase this involved screening additional Leu+ Uracolonies by PCR for those that had arisen by site-specific recombination