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Table 1 Assaying integrase mediated site-specific recombination in yeast S.cerevisiae

From: Comparison and optimization of ten phage encoded serine integrases for genome engineering in Saccharomyces cerevisiae

Integrase

PCR for site specific recombination attL and attR PCR

Sequence for attL (correct sample /checking number)

Sequence for attR (correct sample/checking number)

ΦBT1

9/10+; 10/10+; 10/10; 10/10

15/15

15/15

10/10+; 10/10+; 10/10; 10/10

15/15

15/15

TP901

10/10+; 10/10+; 10/10; 10/10

15/15

15/15

TG1

9/10+; 9/10+; 9/10; 9/10

15/15

15/15

SPBC

10/10+; 10/10+; 10/10; 10/10

15/15

15/15

R4

9/10+; 8/10+; 8/10; 9/10

15/15

15/15

ΦC31

10/10+; 10/10+; 10/10; 10/10

15/15

15/15

BxB1

10/10+; 10/10+; 10/10; 10/10

15/15

15/15

RV

2/10+; 2/10+; 22/95; 2/10

15/15

15/15

MR11

9/10+; 10/10+; 10/10; 10/10

15/15

15/15

Φ370

8/10+; 7/10+; 9/10; 9/10

15/15

15/15

A118

6/10+; 5/10+; 7/10; 7/10

15/15

15/15

ΦC1

0/50+; 0/50+; 0/50; 0/50

NA

NA

ΦK38

9/10+; 8/10+; 10/10; 9/10

15/15

15/15

  1. Table 1 PCR was carried out following transformation to measure the proportion of Leu+ clones that had undergone recombinase mediated cassette exchange. At least 10 clones were analysed from the strains containing the nls + integrase or the nls- integrase. (+ or – superscript respectively). Fifteen PCR products derived from each type of strain were confirmed by DNA sequencing as corresponding to reciprocal and conservative site-specific recombinants. In the case of RV integrase this involved screening additional Leu+ Uracolonies by PCR for those that had arisen by site-specific recombination