Fig. 2

Assaying toxicity of fifteen phage integrases in S. cerevisiae. Serial ten fold dilutions of yeast expressing a native integrase or an integrase tagged at the C terminus with a nuclear localization signal were plated on SD-Trp agar starting at an initial inoculum of 5 x 10⁴ in the presence or absence of doxycycline and cultured for three days before recording growth