Sequence organization of the DNA used to assay integrase activity in Saccharomyces cerevisiae.
a The structure of the URA3 containing integrase assay construct integrated at the LEU2 locus. b The structure of the donor plasmid used to assay integrase mediated cassette exchange and the product of site-specific recombination between this plasmid and the URA3 containing integrase assay construct. c The generic structure of the plasmid used to inducibly express the integrases. The plasmid is based upon the “Tet-off” vector pCM184. d The sequence organization of the attP and attB arrays used in the target and donor plasmids used to assay integrase mediated cassette exchange