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Fig. 1 | BMC Biotechnology

Fig. 1

From: Comparison and optimization of ten phage encoded serine integrases for genome engineering in Saccharomyces cerevisiae

Fig. 1

Sequence organization of the DNA used to assay integrase activity in Saccharomyces cerevisiae. a The structure of the URA3 containing integrase assay construct integrated at the LEU2 locus. b The structure of the donor plasmid used to assay integrase mediated cassette exchange and the product of site-specific recombination between this plasmid and the URA3 containing integrase assay construct. c The generic structure of the plasmid used to inducibly express the integrases. The plasmid is based upon the “Tet-off” vector pCM184. d The sequence organization of the attP and attB arrays used in the target and donor plasmids used to assay integrase mediated cassette exchange

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BMC Biotechnology

ISSN: 1472-6750

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