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Table 3 Effect of metal ions and chemical reagents on TreZa

From: A trehalase from Zunongwangia sp.: characterization and improving catalytic efficiency by directed evolution

The reagent Concentration/Relative activity (%)
1 mM 5 mM 10 mM
K+ 106.64 ± 1.58c 127.44 ± 6.69 94.71 ± 1.58
Ni+ 158.92 ± 0.69 125.97 ± 1.07 112.76 ± 8.14
Mg2+ 92.46 ± 2.38 100.85 ± 1.02 116.15 ± 5.36
Ca2+ 105.27 ± 5.69 134.43 ± 4.20 126.31 ± 7.24
Fe3+ 65.67 ± 0.12 7.17 ± 0.46 4.91 ± 2.15
Co2+ 94.55 ± 3.60 42.23 ± 0.08 38.46 ± 3.30
Cu2+ 47.47 ± 4.58 3.91 ± 2.47 -b
Zn2+ 19.96 ± 2.27 18.86 ± 0.50 14.3 ± 3.44
Ba2+ 105.25 ± 2.67 135.54 ± 3.59 111.94 ± 0.63
EDTA 70.48 ± 5.36 78.75 ± 9.23 44.45 ± 5.26
ATP 155.65 ± 4.78 76.71 ± 6.00 55.75 ± 8.77
ADP 139.02 ± 0.82 9.09 ± 2.02 -b
  1. a:All assays were preformed in the standard conditions and the activity without additional reagent and ions was taken as 100 %; b:unmeasured data; c:Relative activity ± the standard deviation