A trehalase from Zunongwangia sp.: characterization and improving catalytic efficiency by directed evolution

BMC Biotechnology

Table 3 Effect of metal ions and chemical reagents on TreZ^a

The reagent	Concentration/Relative activity (%)
The reagent	1 mM	5 mM	10 mM
K⁺	106.64 ± 1.58^c	127.44 ± 6.69	94.71 ± 1.58
Ni⁺	158.92 ± 0.69	125.97 ± 1.07	112.76 ± 8.14
Mg²⁺	92.46 ± 2.38	100.85 ± 1.02	116.15 ± 5.36
Ca²⁺	105.27 ± 5.69	134.43 ± 4.20	126.31 ± 7.24
Fe³⁺	65.67 ± 0.12	7.17 ± 0.46	4.91 ± 2.15
Co²⁺	94.55 ± 3.60	42.23 ± 0.08	38.46 ± 3.30
Cu²⁺	47.47 ± 4.58	3.91 ± 2.47	-^b
Zn²⁺	19.96 ± 2.27	18.86 ± 0.50	14.3 ± 3.44
Ba²⁺	105.25 ± 2.67	135.54 ± 3.59	111.94 ± 0.63
EDTA	70.48 ± 5.36	78.75 ± 9.23	44.45 ± 5.26
ATP	155.65 ± 4.78	76.71 ± 6.00	55.75 ± 8.77
ADP	139.02 ± 0.82	9.09 ± 2.02	-^b

^a:All assays were preformed in the standard conditions and the activity without additional reagent and ions was taken as 100 %; ^b:unmeasured data; ^c:Relative activity ± the standard deviation

ISSN: 1472-6750