Skip to main content

Table 2 Steady-state kinetic parameters for wild-type TreZ and mutant

From: A trehalase from Zunongwangia sp.: characterization and improving catalytic efficiency by directed evolution

Enzyme K m (mmol l−1) k cat (s−1) k cat /K m (mmol−1 s−1)
Wild-type(TreZ) 0.99 ± 0.04 263.25 ± 0.91 265.91 ± 1.21
C4 0.3793 ± 0.03 433.69 ± 0.82 1143.40 ± 1.67
Y227H 0.7194 ± 0.05 299.83 ± 1.39 416.78 ± 1.57
R442G 0.4317 ± 0.03 319.88 ± 0.95 740.97 ± 1.64
  1. The data are the average of three replicates