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Table 2 Steady-state kinetic parameters for wild-type TreZ and mutant

From: A trehalase from Zunongwangia sp.: characterization and improving catalytic efficiency by directed evolution

Enzyme

K m (mmol l−1)

k cat (s−1)

k cat /K m (mmol−1 s−1)

Wild-type(TreZ)

0.99 ± 0.04

263.25 ± 0.91

265.91 ± 1.21

C4

0.3793 ± 0.03

433.69 ± 0.82

1143.40 ± 1.67

Y227H

0.7194 ± 0.05

299.83 ± 1.39

416.78 ± 1.57

R442G

0.4317 ± 0.03

319.88 ± 0.95

740.97 ± 1.64

  1. The data are the average of three replicates