Fig. 2From: A trehalase from Zunongwangia sp.: characterization and improving catalytic efficiency by directed evolutionEffects of pH and temperature on enzyme activity and stability. a Effect of temperature on the activity of TreZ and mutants. The maximal activity was taken as 100Â %. b Effect of temperature on the stability of TreZ and mutants. The enzyme activity without pre-treatment was taken as 100Â %. c Effect of pH on the activity of TreZ and mutants. The maximal activity was taken as 100Â %. Black line represents WT (TreZ), red line represents C4, green line represents Y227H and grey line represents R442GBack to article page