Single-molecule chromatin mapping with the CpG-specific DNA methyltransferase M.SssI on the EEF1A1 promoter in PT1-CHO cell lines. a Schematic annotation of a predicted nucleosome (designated as Nuc 853) with putative transcription factor binding sites (green, rectangle boxes), and CpGs (gray, square boxes). Below panels are representative M.SssI maps and interpretation obtained in PT1-55. Methylated (= unprotected CpGs, red); Unmethylated (= protected CpGs, blue); white squares are missing or unclear values. b The analyzed nucleosomes nearest the TSS and M.SssI maps showing more protected CpGs in PT1-7 than in PT1-55.